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Metagenomic Analysis of Uterine Microbiota in Postpartum Normal and Endometritic Water Buffaloes (Bubalus bubalis)
ISSN: 1927 - 520X
Publisher: author
Metagenomic Analysis of Uterine Microbiota in Postpartum Normal and Endometritic Water Buffaloes (Bubalus bubalis)
Indexed in
Agriculture and Food Sciences
ARTICLE-FACTOR
1.3
Article Basics Score: 2
Article Transparency Score: 2
Article Operation Score: 2
Article Articles Score: 3
Article Accessibility Score: 2
SUBMIT PAPER ASK QUESTION
International Category Code (ICC):
ICC-0202
Publisher: Lifescience Global Inc.
International Journal Address (IAA):
IAA.ZONE/192710633520X
eISSN
:
1927 - 520X
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Abstract
In Indian subcontinent the water buffalo (Bubalus bubalis) is one of the important livestock animals. As in cows, postpartum infection like endometritis in dairy buffaloes is major cause for the economic loss in the dairy industries. Till date, there is no study regarding metagenomic analysis of bacterial population of postpartum endometritic buffaloes. The purpose of this study was to identify and compare the uterine bacterial composition in normal and endometritic postpartum buffaloes using 16S rDNA cloning, which was a type of culture-independent methods. A total of 151 cloned plasmids for 16S rDNA from both normal and endometritic uterine samples were sequenced. Cloning library of 16S rDNA revealed clear cut difference between bacterial populations of normal and endometritic postpartum buffaloes. Cloned sequences were assigned to five major groups and one uncultured group. The five major groups include- Bacteroidetes, Firmicutes, ...
