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Molecular detection of virulence factors (adhesion genes) in some Staphylococcus epidermidis locally isolated from dif...
ISSN: 0976 - 7614
Publisher: author
Molecular detection of virulence factors (adhesion genes) in some Staphylococcus epidermidis locally isolated from dif...
Indexed in
Biology and Life Sciences
ARTICLE-FACTOR
1.3
Article Basics Score: 2
Article Transparency Score: 2
Article Operation Score: 2
Article Articles Score: 2
Article Accessibility Score: 3
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International Category Code (ICC):
ICC-0402
Publisher: Journal Of Advanced Laboratory Research In Biology Society..
International Journal Address (IAA):
IAA.ZONE/097644657614
eISSN
:
0976 - 7614
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Abstract
The icaA and icaD genes are found in bacterial cells. The icaA and icaD genes allow a bacterium to produce biofilm. Biofilms are an important virulence determinant in bacteria because biofilm formation significantly increases resistance to antibiotics and promotes host defenses. The ability of biofilm production can be determined by two phenotypic methods and genotypic methods of some adhesion factors (icaA and icaD). The phenotypic methods included the Congo-red agar (CRA) and Microtiter plate (Mtp). The results showed that 58 (69%) from 84 isolates were positive for producing biofilm on (CRA) plates and the 55 (65%) from 84 isolates were positive for adhesion and biofilm formation by (Mtp) on the O.D. 490nm. The extraction of DNA is subjected to use for polymerase chain reaction (PCR) which is used to amplify specific genes. The production of viscous layer of Staphylococcus epidermidis was investigated by using Molecular analysis (P...
