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Isolation and characterization of Cysteine protease from leguminous cotyledons
ISSN: 0976 - 7614
Publisher: author
Isolation and characterization of Cysteine protease from leguminous cotyledons
Indexed in
Biology and Life Sciences
ARTICLE-FACTOR
1.3
Article Basics Score: 3
Article Transparency Score: 2
Article Operation Score: 3
Article Articles Score: 3
Article Accessibility Score: 2
SUBMIT PAPER ASK QUESTION
International Category Code (ICC):
ICC-0402
Publisher: Journal Of Advanced Laboratory Research In Biology Society..
International Journal Address (IAA):
IAA.ZONE/097652277614
eISSN
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0976 - 7614
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Abstract
Proteolytic enzymes play central role in the biochemical mechanism of germination and intricately involved in many aspects of plant physiology and development. The present study was conducted on the compares studies the Cysteine proteases from four varieties of 62 hours germinated leguminous seeds: lentil, green gram, black gram and pea bean. We elaborated the easy procedure for isolation of protease from leguminous germinated seeds by using (NH4)2SO4 precipitation followed by Gel-filtration and DEAE-cellulose chromatography from the 72h germinated cotyledons of lentil (Lens esculenta), green gram (Vigna radiata), black gram (Vigna mungo) and pea bean (Phaseolus vulgaris). This study revealed that the water-soluble protein concentration of crude extract ranged between 2.03 to 2.36mg/ml in which green gram was highest protein concentration (2.36mg/ml) and lentil accounted was the lowest concentration (2.03mg/ml). Cysteine proteases fro...
