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A Novel Protocol for Stevia rebaudiana (Bert.) Regeneration
ISSN: 0976 - 7614
Publisher: author
A Novel Protocol for Stevia rebaudiana (Bert.) Regeneration
Indexed in
Biology and Life Sciences
ARTICLE-FACTOR
1.3
Article Basics Score: 2
Article Transparency Score: 3
Article Operation Score: 3
Article Articles Score: 3
Article Accessibility Score: 2
SUBMIT PAPER ASK QUESTION
International Category Code (ICC):
ICC-0402
Publisher: Journal Of Advanced Laboratory Research In Biology Society..
International Journal Address (IAA):
IAA.ZONE/097652367614
eISSN
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0976 - 7614
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Abstract
Stevia rebaudiana Bertoni has sweet substances (stevioside) in its leaves that are free of calories and their consumption is beneficial for diabetic patients and is also helpful in high blood pressure also. Because of low capability in seed germination, tissue culture is an appropriate method for propagation of this plant. In the current study, optimization of stevia in vitro cultivation via direct organogenesis with different explants, light intensities and plant hormones has been examined. These treatments included BAP (at 0.5, 1, 1.5 and 2mg/l) in combination with 2,4-D, IBA and NAA (each with concentrations of 0.1, 0.2 and 0.5mg/l) and different light intensities (Dark, 2000, 4000 and 6000 lux). MS was utilized as a basal medium. Results indicated the highest rate of organogenesis (85%) occurred on the axillary buds explants with a medium containing 1.5mg/l BAP + 0.1mg/l NAA under 6000 lux light intensity. Also, the highest range ...
