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Immobilization Parameters Statistically Optimized for Whole Cells of Pseudomonas putida G7 to Enhance Limonin Biotrans...
ISSN: 0976 - 7614Publisher: author   
Immobilization Parameters Statistically Optimized for Whole Cells of Pseudomonas putida G7 to Enhance Limonin Biotrans...
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Biology and Life Sciences
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1.3
Article Basics Score: 2
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Article Articles Score: 3
Article Accessibility Score: 3
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International Category Code (ICC):
ICC-0402
Publisher: Journal Of Advanced Laboratory Research In Biology Society..
International Journal Address (IAA):
IAA.ZONE/097652447614
eISSN
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0976 - 7614
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Abstract
This study was aimed for optimizing the immobilization parameters for Pseudomonas putida G7 in Ca-alginate beads, in order to establish a debittering strategy for citrus juices, by biotransforming the bitter principle - Limonin. Response Surface Methodology (RSM) with Central Composite Design (CCD) was employed to model the significant parameters for an enhanced response. An enhanced limonin bioconversion and immobilized bead stability was obtained with alginate concentration (2%), cell load (47.2g/l), and a bead diameter (2.1mm); which had significant effects (p