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Optimization of Extracellular Keratinase Production by Aspergillus terreus Isolated from Chicken's Litter
ISSN: 0976 - 7614
Publisher: author
Optimization of Extracellular Keratinase Production by Aspergillus terreus Isolated from Chicken's Litter
Indexed in
Biology and Life Sciences
ARTICLE-FACTOR
1.3
Article Basics Score: 3
Article Transparency Score: 3
Article Operation Score: 2
Article Articles Score: 3
Article Accessibility Score: 3
SUBMIT PAPER ASK QUESTION
International Category Code (ICC):
ICC-0402
Publisher: Journal Of Advanced Laboratory Research In Biology Society..
International Journal Address (IAA):
IAA.ZONE/097652757614
eISSN
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0976 - 7614
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Abstract
In this current study 45 fungal isolates were isolated from chicken's litter on Feather Agar Medium (FAM) were screened for determining the potent keratinase producing isolates. Out of these fungal isolates, twelve species and one species variety exhibited various degrees of keratinolytic activities from which A. terreus showed the highest keratinase production (12.6U/ml). The optimum temperature and initial pH for keratinase production by A. terreus were 40°C and 8, respectively. The highest keratinase production was observed for a period 25 days. The optimum ionic strength for the enzyme production was 80mM NaCl. Deprivation of K+, Fe2+, Mg2+, Ca2+ or Zn2+ from the culture medium drastically reduced the keratinase production by A. terreus. In contrast, sulfur deprivation did not significantly affect the keratinase production. The Km and Vmax values for A. terreus keratinase were 8.64mg keratin and 56.7U/mg proteins, respectively. T...
