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Effect on Physicochemical Characters and Bioactivity of Argemone mexicana in Different Plant Extraction Methods
ISSN: 0976 - 7614Publisher: author   
Effect on Physicochemical Characters and Bioactivity of Argemone mexicana in Different Plant Extraction Methods
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Biology and Life Sciences
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1.3
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Article Articles Score: 2
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International Category Code (ICC):
ICC-0402
Publisher: Journal Of Advanced Laboratory Research In Biology Society..
International Journal Address (IAA):
IAA.ZONE/097653197614
eISSN
:
0976 - 7614
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Abstract
A study was conducted to find out the variation in physicochemical characters and bioactivity in plant extract prepared by two different methods, in order to decide a preferential method. Methanolic extract of Argemone mexicana plant extract was prepared by Soxhlet extraction method and reflux method. Bioactivity of plant extract was ascertained by a brine shrimp lethality test. The percentage yield of methanolic extract was 18% and 42% in soxhlet and reflux extraction methods respectively. In the first method, extract was greenish brown and sticky, but blackish and nonsticky in later method. Soxhlet method showed solubility in ethanol, chloroform, methanol and acetone whereas the reflux method extract did not show solubility in methanol. In both the method, plant extract was positive for alkaloids and saponins tested with standard methods. Nauplii of brine shrimp were hatched in artificial seawater with routine technique and collecte...