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BlaOXA-48 Genotypic Detection of Carbapenem Resistance in Isolates ofPseudomonas Aeruginosa
ISSN: 2617 - 9482
Publisher: author
BlaOXA-48 Genotypic Detection of Carbapenem Resistance in Isolates ofPseudomonas Aeruginosa
Indexed in
Health Sciences
ARTICLE-FACTOR
1.3
Article Basics Score: 2
Article Transparency Score: 3
Article Operation Score: 2
Article Articles Score: 2
Article Accessibility Score: 2
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International Category Code (ICC):
ICC-0702
Publisher: Bahria University Medical And Dental College
International Journal Address (IAA):
IAA.ZONE/261797589482
eISSN
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2617 - 9482
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Abstract
Objective: To determine the prevalence of carbapenem resistance in strains of Pseudomonas aeruginosa at a molecularlevel by detecting OXA-48 gene which transcribe for resistance to the antibiotic carbapenem among indoor patients of atertiary care hospital Karachi.Study Design and Setting: This observational cross-sectional study was conducted from September 2018 to May 2019at PNS Shifa hospital of Karachi.Methodology: Total 140 strains of Pseudomonas aeruginosa were received and cultured from pus samples. These sampleswere collected from different wards like medicine, surgery, burn unit, ICU, ENT, plastic surgery, paedriatic and familyward. Carbapenem resistance was screened phenotypically by AST (Antibiotic susceptibility test), MHT (Modified Hodgetest) and mCIM (Modified Carbapenem Inactivation Method) in all samples. Only in resistant cases OXA-48 gene wasdetected by real time PCR (polymerase chain reaction). Data was analyzed by f...
